Fluorescence Axial Localization with Nanometer Accuracy and Precision
| Title | Fluorescence Axial Localization with Nanometer Accuracy and Precision |
| Publication Type | Journal Article |
| Year of Publication | 2012 |
| Authors | Li H, Yen CF, Sivasankar S |
| Journal Title | Nano Letters |
| Volume | 12 |
| Pages | 3731-3735 |
| Date Published | 07 |
| Type of Article | Article |
| ISBN Number | 1530-6984 |
| Accession Number | WOS:000306296200063 |
| Keywords | atomic force microscope, Axial localization, confocal fluorescence microscope, DNA, DNA conformation, fluorophores, interference-contrast microscopy, limit, optical reconstruction microscopy, PROBES, resolution, single molecule, storm |
| Abstract | We describe a new technique, standing wave axial nanometry (SWAN), to image the axial location of a single nanoscale fluorescent object with sub-nanometer accuracy and 3.7 nm precision. A standing wave, generated by positioning an atomic force microscope tip over a focused laser beam, is used to excite fluorescence; axial position is determined from the phase of the emission intensity. We use SWAN to measure the orientation of single DNA molecules of different lengths, grafted on surfaces with different functionalities. |
| URL | <Go to ISI>://WOS:000306296200063 |
| DOI | 10.1021/nl301542c |
| Alternate Journal | Nano Lett. |
