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Real-time three-dimensional imaging of cell division by differential interference contrast microscopy

TitleReal-time three-dimensional imaging of cell division by differential interference contrast microscopy
Publication TypeJournal Article
Year of Publication2008
AuthorsTsunoda M, Isailovic D, Yeung ES
Journal TitleJournal of Microscopy-Oxford
Volume232
Pages207-211
Date Published11/01
Type of ArticleArticle
ISBN Number0022-2720
Accession NumberISI:000260544500003
Keywords3D live-cell imaging, Cell division, DETECTOR, differential interference contrast microscopy, FABRY-PEROT INTERFEROMETRY, LIQUID-CHROMATOGRAPHY, MICROTUBULE, microtubules, refractive-index, sub-diffraction limit resolution, VISUALIZATION
Abstract

Differential interference contrast (DIC) microscopy can provide information about subcellular components and organelles inside living cells. Applicability to date, however, has been limited to 2D imaging. Unfortunately, understanding of cellular dynamics is difficult to extract from these single optical sections. We demonstrate here that 3D differential interference contrast microscopy has sub-diffraction limit resolution both laterally and vertically, and can be used for following Madin Darby canine kidney cell division process in real time. This is made possible by optimization of the microscope optics and by incorporating computer-controlled vertical scanning of the microscope stage.
DOI10.1111/j.1365-2818.2008.02091.x
Alternate JournalJ. Microsc.-Oxf.