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Atmospheric pressure laser desorption/ionization of plant metabolites and plant tissue using colloidal graphite

TitleAtmospheric pressure laser desorption/ionization of plant metabolites and plant tissue using colloidal graphite
Publication TypeJournal Article
Year of Publication2010
AuthorsPerdian DC, Schieffer GM, Houk RS
Journal TitleRapid Communications in Mass Spectrometry
Volume24
Pages397-402
Date Published02/01
ISBN Number0951-4198
Accession NumberISI:000274585000001
Keywordschromatography, EFFICIENCY, imaging mass-spectrometry, maldi-ms, MATRIX, nanoparticles, peptides, proteins, SMALL MOLECULES, wavelength
Abstract

Colloidal graphite is a promising matrix for atmospheric pressure laser desorption/ionization mass spectrometry. Intact [M+H](+) and [M-H](-) ions are readily produced from a wide range of small molecule plant metabolites, particularly anthocyanins, fatty acids, lipids, glycerides, and ceramides. Compared with a more traditional organic acid matrix, colloidal graphite provides more efficient ionization for small hydrophobic molecules and has a much cleaner background spectrum, especially in negative ion mode. Some important metabolites, e.g., fatty acids and glycosylated flavonoids, can be observed from Arabidopsis thaliana leaf and flower petal tissues in situ. Copyright (C) 2010 John Wiley & Sons, Ltd.
URL<Go to ISI>://000274585000001
DOI10.1002/Rcm.4405