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In situ probing of cholesterol in astrocytes at the single-cell level using laser desorption ionization mass spectrometric imaging with colloidal silver

TitleIn situ probing of cholesterol in astrocytes at the single-cell level using laser desorption ionization mass spectrometric imaging with colloidal silver
Publication TypeJournal Article
Year of Publication2010
AuthorsPerdian DC, Cha S, Oh J, Sakaguchi DS, Yeung ES, Lee YJ
Journal TitleRapid Communications in Mass Spectrometry
Volume24
Pages1147-1154
Date Published04/01
ISBN Number0951-4198
Accession NumberISI:000276775700005
Keywordsablation electrospray-ionization, ARABIDOPSIS-THALIANA, brain-tissue, enzymatic determination, fluorescence microscopy, lipid rafts, maldi-ms, MEMBRANES, PERFORMANCE LIQUID-CHROMATOGRAPHY, SMALL MOLECULES
Abstract

Mass spectrometric imaging has been utilized to localize individual astrocytes and to obtain cholesterol populations at the single-cell level in laser desorption ionization (LDI) with colloidal silver. The silver ion adduct of membrane-bound cholesterol was monitored to detect individual cells. Good correlation between mass spectrometric and optical images at different cell densities indicates the ability to perform single-cell studies of cholesterol abundance. The feasibility of quantification is confirmed by the agreement between the LDI-MS ion signals and the results from a traditional enzymatic fluorometric assay. We propose that this approach could be an effective tool to study chemical populations at the cellular level. Published in (C) 2010 by John Wiley & Sons, Ltd.
URL<Go to ISI>://000276775700005
DOI10.1002/Rcm.4491