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Resolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorods and Differential Interference Contrast Microscopy

TitleResolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorods and Differential Interference Contrast Microscopy
Publication TypeJournal Article
Year of Publication2010
AuthorsWang GF, Sun W, Luo Y, Fang N
Journal TitleJournal of the American Chemical Society
Volume132
Pages16417-16422
Date Published11/24
ISBN Number0002-7863
Accession NumberISI:000284792000032
Keywordscargo transport, f-1-atpase, kinesin, molecular motors, myosin-v, nanoparticles, orientation sensors, single-particle tracking, torque component, tug-of-war
Abstract

Gold nanorods are excellent orientation probes due to their anisotropic optical properties. Their dynamic rotational motion in the 3D space can be disclosed with Nomarski-type differential interference contrast (DIC) microscopy. We demonstrate that by using the combination of gold nanorod probes and DIC microscopy, we are able to resolve rotational motions of nano-cargos transported by motor proteins at video rate not only on engineered surfaces but also on cytoskeleton tracks in live cells.
URL<Go to ISI>://000284792000032http://pubs.acs.org/doi/pdfplus/10.1021/ja106506k
DOI10.1021/Ja106506k
Alternate JournalJ Am Chem Soc