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Raman spectroscopy measurements of glucose and xylose in hydrolysate: Role of corn stover pretreatment and enzyme composition

TitleRaman spectroscopy measurements of glucose and xylose in hydrolysate: Role of corn stover pretreatment and enzyme composition
Publication TypeJournal Article
Year of Publication2011
AuthorsShih CJ, Lupoi JS, Smith EA
Journal TitleBioresource Technology
Volume102
Pages5169-5176
Date Published04
ISBN Number0960-8524
Accession NumberISI:000289703400030
Keywordsacid pretreatment, aqueous ammonia, biofuels, cell-walls, enzymatic hydrolysate, features, fermentation, glucose quantitation, lignocellulosic biomass, phloroglucinol, raman spectroscopy, saccharification, sugars, technologies, xylose quantitation
Abstract

The effect of corn stover pretreatment on glucose quantitation in hydrolysate using Raman spectroscopy is evaluated. Dilute sulfuric-acid pretreatment results in a 20 mg mL(-1) glucose limit of detection in hydrolysate. Soaking in aqueous ammonia pretreatment produces a 4 mg mL(-1) limit of detection. Water, ethanol or hexane extraction of corn stover reduces the spectral background that limits glucose detection in dilute acid hydrolysate. Additionally. a Raman spectroscopy multi-peak fitting method is presented to simultaneously measure glucose and xylose concentration in hydrolysate. This method yields a 6.1% average relative standard error at total saccharide concentrations above 45 mg mL(-1). When only cellulase is present, glucose and xylose yield were measured by Raman spectroscopy to be 32 +/- 4 and 7.0 +/- 0.8 mg mL(-1), respectively. When both cellulase and hemicellulase were present, xylose yield increased to 18.0 +/- 0.5 mg mL(-1). Enzymatic or colorimetric assays confirmed the validity of the Raman spectroscopy results. (C) 2011 Elsevier Ltd. All rights reserved.
URL<Go to ISI>://000289703400030
DOI10.1016/j.biortech.2011.01.043
Alternate JournalBioresource TechnolBioresource Technol