Aptamer Functionalized Microcantilever Sensors for Cocaine Detection
| Title | Aptamer Functionalized Microcantilever Sensors for Cocaine Detection |
| Publication Type | Journal Article |
| Year of Publication | 2011 |
| Authors | Kang K, Sachan A, Nilsen-Hamilton M, Shrotriya P |
| Journal Title | Langmuir |
| Volume | 27 |
| Pages | 14696-14702 |
| Date Published | 12 |
| ISBN Number | 0743-7463 |
| Accession Number | WOS:000297427500092 |
| Abstract | A cocaine-specific aptamer was used as a receptor molecule in a microcantilever-based surface stress sensor for detection of cocaine molecules. An interferometric technique that relies on measuring differential displacement between two microcantilevers (a sensing/reference pair) was utilized to measure the cocaine/aptamer binding induced surface stress changes. Sensing experiments were performed for different concentrations of cocaine from 25 to 500 mu M in order to determine the sensor response as a function of cocaine concentration. In the lower concentration range from 25 to 100 mu M, surface stress values increased proportionally to coverage of aptamer/cocaine complexes from 11 to 26 mN/m. However, as the cocaine concentration was increased beyond 100 mu M, the surface stress values demonstrated a weaker dependence on the affinity complex surface coverage. On the basis of a sensitivity of 3 mN/m for the surface stress measurement, the lowest detectable threshold for the cocaine concentration is estimated to be 5 mu M. Sensing cantilevers could be regenerated and reused because of reversible thermal denaturation of aptamer. |
| URL | <Go to ISI>://WOS:000297427500092 |
| DOI | 10.1021/la202067y |
