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Highly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization

TitleHighly sensitive detection of microRNA by chemiluminescence based on enzymatic polymerization
Publication TypeJournal Article
Year of Publication2012
AuthorsMa CB, Yeung ES, Qi SD, Han R
Journal TitleAnalytical and Bioanalytical Chemistry
Volume402
Pages2217-2220
Date Published02
Type of ArticleArticle
ISBN Number1618-2642
Accession NumberWOS:000300308400023
KeywordsARRAY, cancer, capillary-electrophoresis, cells, CHEMILUMINESCENCE, GENE-EXPRESSION, lna, microarray, MicroRNA, mirnas, rna, rolling-circle amplification, surface
Abstract

We have developed a new methodology for miRNA assay using chemiluminescence imaging by poly(U) polymerase catalyzed miRNA polymerization. This method is very sensitive with a 50 fM limit of detection, which is comparable to or better than current assay methods. Multiplex detection for miRNA can be easily realized by introducing different capture probes onto the biosensor array, which will make it highly versatile for various research purposes.
URL<Go to ISI>://WOS:000300308400023
DOI10.1007/s00216-011-5653-4
Alternate JournalAnal. Bioanal. Chem.