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Parameters Affecting the Efficient Delivery of Mesoporous Silica Nanoparticle Materials and Gold Nanorods into Plant Tissues by the Biolistic Method

TitleParameters Affecting the Efficient Delivery of Mesoporous Silica Nanoparticle Materials and Gold Nanorods into Plant Tissues by the Biolistic Method
Publication TypeJournal Article
Year of Publication2012
AuthorsMartin-Ortigosa S, Valenstein JS, Sun W, Moeller L, Fang N, Trewyn BG, Lin VSY, Wang K
Journal TitleSmall
Volume8
Pages413-422
Date Published02
Type of ArticleArticle
ISBN Number1613-6810
Accession NumberWOS:000299621500012
Keywordsbiolistic method, DNA delivery, DOTS, high-velocity microprojectiles, LIVING CELLS, maize, mesoporous silica, nanorods, nucleic-acids, plants, protoplasts, pumpkin plants, quantum, size, tobacco, translocation
Abstract

Applying nanotechnology to plant science requires efficient systems for the delivery of nanoparticles (NPs) to plant cells and tissues. The presence of a cell wall in plant cells makes it challenging to extend the NP delivery methods available for animal research. In this work, research is presented which establishes an efficient NP delivery system for plant tissues using the biolistic method. It is shown that the biolistic delivery of mesoporous silica nanoparticle (MSN) materials can be improved by increasing the density of MSNs through gold plating. Additionally, a DNA-coating protocol is used based on calcium chloride and spermidine for MSN and gold nanorods to enhance the NP-mediated DNA delivery. Furthermore, the drastic improvement of NP delivery is demonstrated when the particles are combined with 0.6 mu m gold particles during bombardment. The methodology described provides a system for the efficient delivery of NPs into plant cells using the biolistic method.
URL<Go to ISI>://WOS:000299621500012http://onlinelibrary.wiley.com/store/10.1002/smll.201101294/asset/413_ftp.pdf?v=1&t=gzplp2gg&s=56e0b631b39b3c1216c46762f8592268492768d3
DOI10.1002/smll.201101294
Alternate JournalSmall