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Highly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization

TitleHighly sensitive detection of alkaline phosphatase using molecular beacon probes based on enzymatic polymerization
Publication TypeJournal Article
Year of Publication2012
AuthorsMa CB
Journal TitleMolecular and Cellular Probes
Volume26
Pages113-115
Date Published06
Type of ArticleArticle
ISBN Number0890-8508
Accession NumberWOS:000303701600002
KeywordsAlkaline phosphatase, assay, Inhibitor, Molecular beacon, okadaic acid, SUBSTRATE
Abstract

We have developed a new methodology for highly sensitive alkaline phosphatase assay using molecular beacon probes. No incubation step is needed to obtain a limit of detection for ALP of 2 x 10(-16) M. Furthermore, ALP inhibition by the inhibitor okadaic acid is shown, demonstrating the potential for high-throughput screening for inhibitors. (C) 2012 Elsevier Ltd. All rights reserved.
URL<Go to ISI>://WOS:000303701600002
DOI10.1016/j.mcp.2012.03.005
Alternate JournalMol. Cell. Probes