Detecting and Tracking Nonfluorescent Nanoparticle Probes in Live Cells
| Title | Detecting and Tracking Nonfluorescent Nanoparticle Probes in Live Cells |
| Publication Type | Book Chapter |
| Year of Publication | 2012 |
| Authors | Wang GF, Fang N |
| Editor | Conn PM |
| Book Title | Imaging and Spectroscopic Analysis of Living Cells: Optical and Spectroscopic Techniques |
| City | San Diego |
| Publisher | Elsevier Academic Press Inc |
| Volume | 504 |
| Pages | 83-108 |
| Series Title | Methods in Enzymology |
| ISBN Number | 0076-6879978-0-12-391857-4 |
| Accession Number | WOS:000303896400004 |
| Keywords | dark-field, gold nanorods, interference contrast microscopy, LIVING CELLS, microscopy, molecular motors, nanometer spatial precision, scattering, single-particle tracking, transient confinement zones, tug-of-war |
| Abstract | Precisely imaging and tracking dynamic biological processes in live cells are crucial for both fundamental research in life sciences and biomedical applications. Nonfluorescent nanoparticles are emerging as important optical probes in live-cell imaging because of their excellent photostability, large optical cross sections, and low cytotoxicity. Here, we provide a review of recent development in optical imaging of nonfluorescent nanoparticle probes and their applications in dynamic tracking and biosensing in live cells. A brief discussion on cytotoxicity of nanoparticle probes is also provided. |
| URL | <Go to ISI>://WOS:000303896400004 |
| DOI | 10.1016/b978-0-12-391857-4.00004-5 |
| Abbreviation | Methods Enzymol. |
