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Fluorescence detection of adenosine triphosphate using smart probe

TitleFluorescence detection of adenosine triphosphate using smart probe
Publication TypeJournal Article
Year of Publication2012
AuthorsMa CB, Chen HC, Han R, He HL, Zeng WM
Journal TitleAnalytical Biochemistry
Volume429
Pages8-10
Date Published10
Type of ArticleArticle
ISBN Number0003-2697
Accession NumberWOS:000308271700002
KeywordsACID, assay, ATP, fluorescence detection, ligation, molecular beacons, recognition, signal, Smart probe, T4 DNA ligase
Abstract

A novel fluorescent probe for adenosine triphosphate (ATP) assay based on DNA ligation is proposed in this article. This approach uses a novel smart probe, T4 DNA ligase, and two short oligonucleotides. In the presence of ATP, the 14 DNA ligase catalyzes the ligation reaction and the ligation product restores the fluorescence of the smart probe. This method is very sensitive with a 0.5-nM limit of detection. Compared with current assay methods, the strategy is simpler, cheaper, and 40 times more sensitive. (C) 2012 Elsevier Inc. All rights reserved.
URL<Go to ISI>://WOS:000308271700002
DOI10.1016/j.ab.2012.06.022
Alternate JournalAnal. Biochem.